Skip to main content
. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Cell. 2013 Feb 28;152(5):1021–1036. doi: 10.1016/j.cell.2013.01.052

Figure 7. TAF3 Interacts with H3K4me3 to Facilitate the Rapid Induction of Selective p53 Target Genes.

Figure 7

(A) HCT116 cells were transfected with non-targeting control (control) or WDR5 siRNA and treated with doxorubicin for 0 or 24 h. Immunoblot analysis of cell lysates with the indicated antibodies. (B) QRT-PCR analysis of p53 target genes in HCT116 cells that were treated as in (A). The expression levels determined after doxorubicin are relative to the levels before doxorubicin. Error bars denote the standard deviation from duplicate reactions by real time PCR. (C, D) ChIP analysis of HCT116 cells transfected with control or WDR5 siRNA and treated with doxorubicin for 0 or 8 h. The levels of H3K4me3 are relative to H3 levels. Schematic showing the amplicons used for qRT-PCR. An average of two independent ChIP experiments that are representative of at least three is shown with error bars denoting the standard deviation. (E) (left) QRT-PCR and (right) immunoblot analysis of TAF3 in HCT116 cells that were transfected with control or TAF3 siRNA together with empty vector control or siRNA-resistant plasmids expressing wild-type or M880A mutant TAF3. The expression levels determined after doxorubicin are relative to the levels before doxorubicin and error bars denote the standard deviation from duplicate reactions by real time PCR. (F) QRT-PCR analysis of p53 target gene expression in HCT116 cells treated as described in (E). Error bars denote the standard deviation from duplicate reactions by real time PCR.