Figure 2.

Quantitative analysis of cysteine specific labeling in the glutathione deficient mutant pad2-1, the complemented line OE3 and after the treatment of wild type plants with buthionine sulfoximine (BSO). Graph shows means with standard errors and documents changes in the density of gold particles bound to cysteine in the respective Arabidopsis leaf cells when compared to control wildtype plants. Significant differences were calculated using the Mann-Whitney U-test; (***) indicates significance at the 0.001 level of confidence. P > 0.05 was considered as not significant (ns); n > 20 for peroxisomes and vacuoles and n > 60 for all other cell structures.