Fig. 7.

Overlap analysis between the MSI-detected molecules and different tumor regions. The necrotic region was separated from the viable region. The latter was further divided into hypoxic and normoxic tumor regions. The necrotic, hypoxic, and normoxic regions together make up the entire tumor tissue. PC(16:0/16:0) was primarily detected from normoxic tumor regions, whereas LPC(16:0/0:0) was characteristic of necrotic tissue. The remaining PCs, SM, and acylcarnitines were colocalizing primarily with hypoxic tumor regions and, to a lesser extent, with normoxic tumor regions. Data are shown as mean ± standard error.