Fig. 4.

Effect of FIC1 and PLD2 loss of function on human BSEP promoter activity in HepG2 cells. HepG2 cells were transfected with the human BSEP promoter as a readout of FXR activity. Activity in untreated HepG2 cells was set at 100% and all results were normalized to renilla luciferase from a pRL-TK construct as a control for transfection efficiency. Cells were then untreated (control) or treated with siPLD2, siFIC1, siPLD2 + siFIC1, or a scrambled siRNA control. The scale of the y axis is linear. Error bars represent the SD for nine measurements. Silencing either PLD2 or FIC1 resulted in a statistically significant reduction in BSEP promoter activity.