Fig. 6.

Effect of FIC1 overexpression in UPS cells. UPS cells were untransfected (−) or transfected with wild-type FIC1 (wt), a Byler disease FIC1 construct (By), or a BRIC disease FIC1 construct (BR). A: Western blot analysis. Crude membranes were probed by Western blot. B: PLD enzyme activity. Depiction of statistical analysis. Mean PLD activity for each treatment is depicted by the bar, with error bars representing SD. PLD activity was highest in the UPS cells transfected with wild-type FIC1. BRIC-transfected cells had an intermediate level of activity, whereas untreated and Byler-transfected cells were equal.