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. 2013 Feb;54(2):457–466. doi: 10.1194/jlr.M032219

Fig. 3.

Fig. 3.

Inhibitor studies of Dictyostelium FAAH. Saturation curves of the reaction of Dictyostelium FAAH hydrolyzing arachidonoyl p-nitroaniline to arachidonic acid and p-nitroaniline with four different inhibitors at three different inhibitor concentrations. Lines of best fit determined by nonlinear regression of either a competitive or noncompetitive inhibition model and errors bars represent ± 1 SD. Inset: Lineweaver-Burk plot of inhibition of Dictyostelium FAAH. A: MAFP (squares, 0 µM; triangles, 100 nM inverted triangles, 1 µM; diamonds 2.5 µM). B: CAY10435 (squares, 0 µM; triangles, 100 nM; inverted triangles, 1 µM; diamonds, 2.5 µM). C: ATFMK (squares, 0 µM; triangles, 14 µM; –inverted triangles, 70 µM; diamonds, 140 µM). D: PMSF (squares, 0 mM; triangles, 1 mM; inverted triangles, 5 mM; diamonds, 10 mM).