Fig. 4.
Multiple reaction monitoring (MRM) chromatograms for each of the seven putative NAEs identified in D. discoideum. The sample was obtained from D. discoideum culture that had been grown to a density of 5 × 106 cells and then exposed to MAFP at a concentration of 10 µM for 24 h. A: Chromatograms from a sample that was not spiked with a 1 nmol mixture of each NAE. B: Chromatograms from a sample from the same culture spiked with a 1 nmol mixture. Elution times are denoted in minutes, and MRM transitions are indicated for each run.