TABLE 3.
Substrate consumption rate of Dictyostelium FAAH on NAE substrates at 300 µM substrate concentration and comparison of acyl chain length on FAAH reaction rates for three different species (n = 3)
| Relative Rate to NAE20:4 |
||||
| Substrate | Substrate Consumption Rate | Dictyostelium | Arabidopsis (35) | Rata (36) |
| nmol/min/mg | ||||
| N-arachidonoylethanolamine (NAE20:4) | 111.4 (58.9)b | 1.00 | 1.00 | 1.00 |
| N-linoleoylethanolamine (NAE18:2) | 83.4 (58.5) | 0.75 | 0.79 | 0.33 |
| N-oleoylethanolamine (NAE18:1) | 112.2 (56.3) | 1.01 | n.d. | 0.32 |
| N-stearoylethanolamine (NAE18:0) | 39.9 (18.7) | 0.36 | n.d. | 0.22 |
| N-palmitoylethanolamine (NAE16:0) | 10.1 (0.5) | 0.09 | 0.68 | 0.23 |
| N-myristoylethanolamine (NAE14:0) | 20.7 (6.6) | 0.19 | 0.51 | 0.27 |
n.d., not determined.
a
In this study, corresponding amide substrates were used (i.e., arachidonamide).
b
Numbers in parentheses represent standard deviations.