Figure 1.

Schematic representation of the extrinsic apoptosis model EARM1.3. Binding of death ligand TRAIL, formation of death-inducing signaling complex (DISC), cleavage of caspases-3, -6 and -8 (C3, C6 and C8), formation of mitochondrial pores, assembly of the apoptosome and cPARP cleavage are shown. Activating interactions such as caspase cleavage and induction of conformational changes are shown as sharp-tipped arrows; inhibitory interactions by competitive binding of proteins such as FLIP, BCL2 and XIAP are shown as flat-tipped arrows. The three fluorescent reporters IC-RP, EC-RP and IMS-RP used in experiment are denoted as yellow lozenges. Specific sets of reactions are called out in red boxes and are keyed to features discussed in subsequent figures.