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β-Glucosidase noncontiguous chimera design chosen for construction. (a) Numbered sequence alignment of the eukaryotic (top) and prokaryotic (bottom) β-glucosidases. Conserved residues are in gray, the block of eukaryotic mutations are in red, and the block of prokaryotic mutations are in green. (b) The two-block design illustrated on the structure of the prokaryotic enzyme, TmBglA (2WBG.pdb).
