Figure 3.

The optimal noncontiguous design breaks far fewer contacts than random two-block partitions of the structure. (a) A histogram of the SCHEMA energies of 10,000 random two-block chimeragenesis designs. The SCHEMA energy of the optimized noncontiguous design is highlighted with a red arrow. (b) The SCHEMA contact map for the optimized noncontiguous two-block design. Most of the SCHEMA contacts are within the two blocks and thus are not disrupted on recombination. The numbering is based on the parent alignment, and SCHEMA contacts are shown in black. Red and green areas show the two blocks (For greater clarity, the conserved residues have been assigned to one of the two blocks based on structural proximity.)