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. 2013 Mar;182(3):1021–1030. doi: 10.1016/j.ajpath.2012.11.022

Figure 1.

Figure 1

AMPK activation by AICAR attenuates LPS-induced lung vascular leakage. AICAR (500 mg/kg i.p.) was injected 4 hours before injection of LPS (1 mg/kg intratracheal); BALF and lung tissue extracts were collected 16 hours later. A: Phosphorylation of AMPK at Thr172 in lungs. B and C: Cell counts and protein concentration in BALF. D: Immunochemical evaluation of lung tissue by H&E staining (original magnification ×40). E: Lung vascular permeability was assessed by accumulation of Evans Blue dye in the lungs. Lungs were excised after perfusion and imaged. Each panel shows the right and left lungs (at right and left, respectively). Spectrophotometric analysis of Evans Blue–stained albumin content in the lung tissues was quantified. Data are expressed as means ± SEM. n = 4 to 6 per group. *P < 0.05 versus control; †P < 0.05 versus LPS. Con, control.