Figure 3.

Circular strains retaining subtelomeric sequences that bind Taz1 show proper meiotic SPB behaviour. Green patches on chromosome diagrams represent retained subtelomeric sequences. (A) When extensive subtelomeric sequences are retained at the fusion points of circular chromosomes [29], Taz1 binding, meiotic bouquet formation and proper meiotic SPB division and separation occur (B,C). Chromatin is viewed through endogenous tagging of one copy of the gene encoding histone H3 (see Methods). SPB, spindle pole body.