Figure 2.

The Atg12—Atg5–Atg16L1 complex regulates the stability of the ULK1–Atg13–FIP200 complex. (A) Immunoblot analysis of Atg proteins in the indicated MEFs. (B) m5-7 cells were cultured in regular medium containing 10 ng/ml Dox for 8 days, and then cultured in the absence of Dox for a further 2 days. (C,D) m5-7 cells were cultured in regular medium with or without 10 ng/ml Dox for 4 days, and then cultured in the presence of 50 μg/ml CHX for the indicated time periods (C). mRNA levels of the indicated genes were measured by quantitative PCR following the 4-day Dox treatment. Data represent mean±s.e. (*P<0.05) (D). CHX, cycloheximide; Dox, doxycycline; MEFs, mouse embryonic fibroblasts; mRNA, messenger RNA; WT, wild-type.