Skip to main content
. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: Nat Cell Biol. 2012 Sep 16;14(10):1105–1112. doi: 10.1038/ncb2578

Figure 1.

Figure 1

WFS1 is required for insulin production, storage and secretion in β-cells. (a) Relative expression of Ins1 and Ins2 measured by quantitative PCR (Ins2 represented in figure; similar for Ins1; n = 3) in rat islets transduced with either GFP or WFS1 lentivirus and incubated with 16.7 mM glucose (16.7G) for 2 h. (b) Pro-insulin biosynthesis measured by immunoblot analysis of immunoprecipitated 35S-labelled insulin in rat islets prepared as in a. 35S-labelled insulin was quantified with Image J and is expressed in arbitrary units (a.u.; n = 3). c, Insulin content measured in rat islets prepared as in a (n = 3). (d) Insulin release measured in rat islets prepared as in a and normalized to insulin content (n = 3). **P < 0.01 when compared with GFP (16.7G). (e–g) Relative expression of BiP (e), sXBP1 (f) and CHOP (g) measured by quantitative PCR and normalized to 2.5G (dashed line) in rat islets prepared as in d (n = 3). (h) Insulin release measured in human islets prepared as in a (n = 3). **P < 0.01 when compared with GFP (16.7G). (i) Relative expression levels of Ins1 and Ins2 measured by quantitative PCR (Ins2 represented in figure; similar for Ins1; n = 3) in INS-1 832/13 pTetR cells (Control), expressing the WFS1 disease mutant P724L or shRNA against Wfs1 and expressing the following WFS1 disease mutants: WFS1-P724L, WFS1G695V or ins483/ter544 (WFS1-ter544). **P < 0.01 when compared with control. (j) Insulin release measured in the same cell lines as i, treated with 16.7G and normalized to insulin content (n = 3). (k) Relative expression of Ins1 and Ins2 measured by quantitative PCR (Ins2 represented in figure; similar for Ins1) in Wfs1+/+ (WT) and Wfs1−/− (KO) mouse islets (n = 3). (l) Insulin content measured in WT and KO islets treated with 16.7G for 2 h (n = 3). (m) Insulin release measured in WT and KO islets treated as in l and normalized to insulin content (n = 3). (n) Insulin release measured in WT and KO islets with or without lenti-WFS1 and treated with 2.5G or 16.7G for 2 h. Insulin levels were normalized to total DNA content (n = 3). All data are means ± s.d. *P < 0.05, **P < 0.01.