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. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: Nat Commun. 2012;3:1000. doi: 10.1038/ncomms2008

Fig. 1. FOXO3 interacts with the ATM-Chk2-p53-H2AX complex.

Fig. 1

(a) Whole cell lysates of MCF-7 treated with CPT (1 μM) for 2 or 4 hours (h) or DMSO (0, control) were subjected to immunoprecipitation (IP) with an anti-FOXO3 antibody (Ab) or an isotype IgG (control) followed by immunoblotting (IB) with Abs as indicated, or an anti-FOXO3 (IP control) or a control Ab against IgG light chain (L). (b–h) Similarly, cell lysates were subjected to IP with an Ab against ATM-pS1981 (b) or p53-pS15 (c) or p53-pS20 (d) or p53-pS46 (e) or Chk2 (f) or γ-H2AX (g) or HIPK2 (h) or an isotype IgG followed by IB with the indicated Abs or a control Ab against IgG(L) or heavy chain (H).