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. Author manuscript; available in PMC: 2013 Mar 5.
Published in final edited form as: Nat Commun. 2012;3:1000. doi: 10.1038/ncomms2008

Fig. 3. FOXO3 is required for ATM-Chk2-p53 phosphorylation and PARP-1 cleavage.

Fig. 3

(a) MCF-7 stable cell lines transfected with FOXO3-shRNA or control-shRNA were treated with CPT (1 μM) or DMSO (negative control) for 4 h, and whole cell lysates were prepared and the levels of proteins were analyzed by IB with specific Abs as highlighted or an anti-β-actin (loading control). (b, c) A549 (b) and H1299 (c) stable cell lines transfected with FOXO3-shRNA or control-shRNA were treated with CPT (1 μM) or DMSO (negative control) for 4 h, and whole cell lysates were analyzed by IB with specific Abs as indicated or an anti-β-actin. (d) Cell lysates from MCF-7 stable cell lines (FOXO3-shRNA and control-shRNA) treated with CPT (1 μM) for an indicated time course were subjected to IB analysis using Abs against PARP-1 and β-actin (loading control).