Skip to main content
. 2013 Jan 15;21(3):570–579. doi: 10.1038/mt.2012.278

Figure 4.

Figure 4

In vivo inhibition of O6-methylguanine-DNA methyltransferase (MGMT) protein expression in human LN18 glioma xenografts through lentivirus delivery of small hairpin RNA (shRNA) targeting MGMT. (a) LN18 wild-type cells were xenografted in nude mice (four per animal). The lentiviral vector expressing the luciferase reporter gene and the shMGMT2 sequence (LV-CMVLuc-shMGMT2) was injected into the left tumors (ntumor = 4 in nmouse = 4). The lentiviral vector expressing the luciferase reporter gene and the control shRNA sequence (LV-CMVLuc-shControl) was injected into the right tumors (ntumor = 4 in nmouse = 4). Bioluminescence emission was measured from 6 to 48 hours after virus intratumoral injection. (b) The bioluminescence emitted by the xenografts was quantified (n = 4 per group). (c) Two days after virus injection, mice were sacrificed and MGMT expression was measured by immunostaining. All data are presented as mean + SD. Bars = 200 µm. CMV, cytomegalovirus.