Skip to main content
. 2012 May 15;122(6):2165–2175. doi: 10.1172/JCI61380

Figure 3. Regulation of SYK(L) by CHK1 occurs via the ubiquitin/proteasome pathway.

Figure 3

(A and B) Huh7 cells were cotransfected with the indicated plasmids for 20 hours and were treated with or without MG132 (10 μM) for 4 hours. The cell lysates were resolved directly by SDS-PAGE or were first incubated with Flag agarose and then analyzed by Western blot. (C) Huh7 cells stably expressing SYK(L) WT or the S295A mutant were incubated with 20 μg/ml of CHX for the indicated times. Cell lysates were then analyzed by Western blot (left panel), and the densities of the SYK(L) protein bands at each time point were normalized to GAPDH and were calculated into percentages using 100% as the value of the zero time point (right panel). (D) Huh7 cells transfected with scrambled siRNA or CHK1 siRNA were incubated with 20 μg/ml of CHX for the indicated times. Cell lysates were then analyzed as described in C.