FIG. 4.

Fourteen consecutive daily treatments of neonatal mice with WIN 18,446 generates testis tubules containing only Sertoli cells and undifferentiated spermatogonia. ZBTB16 and STRA8 localization in testis cross-sections from vehicle control animals (A, B) and animals treated with WIN 18,446 for 14 consecutive days, beginning at 2 dpp (C, D). Black arrows denote ZBTB16-positive spermatogonia, red arrows denote STRA8-positive spermatogonia, and yellow arrows denote STRA8-negative spermatogonia. All bars = 50 μm.