Expression of genes in response to Dmrt1 loss. Espin, Ocln, Cldn11, Tjp, Gata1, Ar, and Krt18 mRNA levels were quantified by quantitative RT-PCR using RNA isolated from testes of Dmrt1+/+ (black bar), Dmrt1−/− (white bar), and Dmrt1−/−;tg (gray bar) mice at P7. *P < 0.05, **P < 0.005, and ***P < 0.0005 (two-tailed Student t-test).