Figure 1. F1G4-IT inhibits protein synthesis in MCF-7 and HepG2 cells but not in KB cells.

Cells were treated with F1G4-IT or mAb VU1D9-rABRa-A for 8 h in leucine free RPMI. The cells were pulsed with ³[H] L-leucine for 2 h and total cellular protein precipitated using 5% TCA. The incorporated radioactivity was plotted against that of the control cells. A: MCF-7; B: HepG2; C: KB. D: Cells treated with F1G4-IT or F1G4-IT_R167L. Each bar represents the mean of three separate experiments carried out with duplicate samples. **P<0.05.