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. 2013 Mar 5;8(3):e58283. doi: 10.1371/journal.pone.0058283

Figure 1. Snf1 activity is required for protection against selenite.

Figure 1

(A) Growth assays of serial dilutions of the respective strains on YPD medium with the indicated additions. Growth was recorded after 48 hours at 30°C. Strains employed: wild type (W303-1A), Δsnf1 (Wsnf1) and Δaft1 (MML348). (B) Growth assays of serial dilutions of the following strains, plated on SC medium with sodium selenite: wild type (W303-1A) and Δsnf1 (Wsnf1) cells transformed with vector pWS93, and Δsnf1 cells transformed with pWS-Snf1, pWS-Snf1-T210A and pWS-Snf1-K84R. Growth was recorded after 3 days at 30°C. (C) As in (A), with the following strains in addition to wild type and Δsnf1: Δsip1Δsip2 (MML1445), Δsip1Δgal83 (MML1452), Δsip2Δgal83 (MML1454), Δsip1Δsip2Δgal83 (MML1459) and Δsnf4 (MML1407).