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. 2013 Mar 5;8(3):e58283. doi: 10.1371/journal.pone.0058283

Figure 4. Phosphorylation levels of Snf1 at Thr210 do not correlate with protection against selenite treatment.

Figure 4

(A) Western blot analysis of phosphorylated Snf1 at Thr210 with anti-phospho-Thr172-AMPK (upper panel). Blots were rehybridized with anti-Snf1 antibodies for total Snf1 (lower panel). Samples were obtained from wild type (W303-1A) exponential cultures in YPD treated with sodium selenite for the indicated times. Control samples were run from YPD-grown wild type cells that were shifted for 1 hour to YPGly (-Glu). (B) Growth assays of serial dilutions of wild type (W303-1A) cells in YPGal medium with the indicated concentrations of selenite. (C) As in (A) with samples from wild type and Δreg1 (MML1442) cells. (D) Growth assays of serial culture dilutions of wild type (W303-1A), Δsnf1 (Wsnf1) and Δreg1 (MML1442) strains on YPD medium with selenite.