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. 2013 Mar 5;8(3):e57630. doi: 10.1371/journal.pone.0057630

Figure 6.YpkA. expression affects endocytosis and vacuolar distribution in A. nidulans.

Figure 6.YpkA

(A) Conidiospores were grown in glass-bottom dishes (Mattek Corporation, USA) with 2 ml of MM-G+supplements for 12 hours at 44°C and exposed to pre-warmed media containing 10 µM FM4-64 for 2 minutes. The coverslips were briefly rinsed in fresh media and incubated in MM-G without FM4-64 for 3 hours to allow for dye internalization and vesicle trafficking. The living cells were visualized at room temperature using a fluorescence microscope. (B, C, and D) show incubation of the FM4-64 for 10, 30, and 60 minutes. Arrows show the Spitzenkörper. Bars, 5 µm.