Table 2. Total length of tubular complexes, total size of tubular complexes and number of junctions after the transfection of endothelial cells HMEC-1 with 3 different siRNA molecules (h_siRNA 529, h_siRNA 240, h_siRNA 241).
Total length of tubular complexes | Total size of tubular complexes | Number of junctions | |||||||
Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | |
HMEC-1 | 103±2 | 96±3 | 104±2 | 116±4 | 94±4 | 96±3 | 100±3 | 93±4 | 106±3 |
h_siRNA 529 | 100±2 | 66±4* | 75±7* | 106±2 | 70±3* | 70±5* | 96±2 | 61±7* | 71±7* |
h_siRNA 240 | 101±2 | 107±4 | 113±4 | 109±5 | 111±4 | 114±4 | 99±1 | 106±6 | 111±3 |
h_siRNA 241 | 95±4 | 103±4 | 109±5 | 105±10 | 109±7 | 112±5 | 90±2 | 101±6 | 108±9 |
siRNA Ctrl | 91±4 | 103±2 | 102±5 | 92±7 | 107±2 | 102±6 | 89±3 | 100±3 | 100±6 |
Data represent arithmetic mean±SEM normalized to cells treated with Lipofectamine RNAiMAX only (% of Lipofectamine RNAiMAX); *P<0.05 vs. untreated control cells (HMEC-1), Lipofectamine RNAiMAX and negative control siRNA (siRNA Ctrl).