Figure 4. SDF-1β increases the number of surviving BMSCs following exposure to H₂O₂.

Cell number of trypan blue-stained A) Tet-Off-SDF-1β BMSCs and B) Tet-Off-EV control BMSCs after vehicle control or H₂O₂ treatment. SDF-1β significantly increased the number of surviving cells (trypan blue negative) and decreased the number of dying cells (trypan blue positive) in response to H₂O₂ treatment compared to Dox-suppressed and Tet-Off-EV controls (6 h, ±100 ng/ml Dox, ±1.0 mM H₂O₂, ***p<0.0001, −Dox; H₂O₂ vs. +Dox; H₂O₂, n = 3, 3 independent experiments).