Figure 4. Molybdate accumulation in vivo. A.
, Time course of RF530:F480 in living animal cells at 37°C responded to different doses of molybdate. HEK-293T transfected with MolyProbe was treated by molybdate at the concentrations indicated. Fluorescence of the cell was measured at 0, 0.5, 1, 2, 3, 4 hr, and RF530:F480 calculated. B, Effect of medium exchange on the time course. The cell medium was replaced by fresh D-MEM/F-12/FBS medium prior to the assay. C, Time course of RF530:F480 in the presence of 10 mM oxalate. D, Time course of RF530:F480 supplemented with sulfate (1 mM). Concentrations of molybdate in the working medium are as follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.