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. 2013 Mar 5;8(3):e58609. doi: 10.1371/journal.pone.0058609

Figure 3. Twistnb expression under differential experimental conditions.

Figure 3

Representative experiments showing non-specific effect of miR-3074-5p and/or miR-691 on both isoforms of Twistnb are shown at a low dose transfection of miRNA precursors. A. Twistnb 3′UTRs at 24 hours post-transfection with miR-3074-5p, miR-691 or both miRNAs. B. Twistnb 3′UTRs at 48 hours post-transfection with miR-3074-5p, miR-691 or both miRNAs. pGL3, pGL3 luciferase vector without insert; NIH, NIH/Ola 3′UTR; SPRET, SPRET/Outbred 3′UTR; NC, scrambled control miRNA; Dark Gray bars, pGL3 luciferase vector; Black bars, pGL3 vector with the NIH/Ola 3′UTR; Light gray bars, pGL3 vector containing the SPRET/Outbred 3′UTR.