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. 2013 Mar 5;8(3):e58615. doi: 10.1371/journal.pone.0058615

Figure 7. Hydrolytic activity of native SERCA.

Figure 7

Enzymatic activity of native SERCA determined in the presence of 10 µM free calcium (•, ▴, X) and in the presence (•, X) or in the absence (▴) of 2 µM A23187. As control experiments, Pi release was also quantified in the absence of calcium ions (○) or in the presence of 1 µM TG (X). Solid lines represent the linear fitting (Y = A+BX) of the experimental data: A =  3 ± 1 µmol Pi/mg; B =  4.4 ± 0.3 µmol Pi/(mg·min) (+A23187); A =  0.6 ± 0.3 µmol Pi/mg; B =  0.68 ± 0.08 µmol Pi/(mg·min) (-A23187). Error bars represent the standard deviations of three measurements. Elapsed time: 30 minutes.