. 2013 Mar 5;8(3):e58615. doi: 10.1371/journal.pone.0058615

Table 1. Optimization of experimental conditions.

Reagent	Solution composition	Concentration range	Optimal concentration range
H₂SO₄	0.48 mM ammonium-heptamolybdate	12.5–625 mM	100–140 mM
	6 mM ascorbic acid
	40 µM tartrate
Ammonium heptamolybdate	125 mM H₂SO₄	0.048–4.8 mM	0.35–0.70 mM
	6 mM ascorbic acid
	40 µM tartrate
Ascorbic acid	125 mM H₂SO₄	0.1–100 mM	>3 mM
	0.48 mM ammonium heptamolybdate
	40 µM tartrate
Tartrate	125 mM H₂SO₄	0.88–400 µM	20–100 µM
	0.48 mM ammonium heptamolybdate
	6 mM ascorbic acid

Experimental conditions employed during the optimization procedure.