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. 2013 Mar 5;8(3):e58580. doi: 10.1371/journal.pone.0058580

Figure 3. Immunohistochemistry of vasohibin-1 after device implantation.

Figure 3

The immunohistochemistry results of vasohibin-1 after NVDD, 10VDD, and pellet implantation are shown. No immunoreactivity was observed after NVDD transplantation (A) and negative control without first antibody (D). 10VDD shows vasohibin-1 immunoreactivity at the device implant area (B). White arrows show the immunoreactivity in the retina and optic nerve at low magnification. Diffuse immunoreactivity was observed in the sclera, choroid, RPE, and retina at greater magnification (E). Strong immunoreactivity was observed in the ganglion cell layer (GCL) and retinal pigment epithelium (RPE), as well as in the sclera and choroid. INL and ONL indicate the inner and outer nuclear layers. These results were not observed in the NVDD group (A) or the negative controls (D and F). Strong immunoreactivity was observed in the pellet (asterisk) and in the tissues surrounding the implanted pellet (C). Yellow arrows indicate the positions where devices or pellets were placed. Devices were removed before sectioning, but pellets were not removed before sectioning. Bars: 200 μm (A–D), and 50 μm (E, F).