Figure 3. Effect of LPIAT1 knockout on liver phosphoinositide lipid molecular species.

Livers from 13 day old littermates expressing (LPIAT1^+/+ (WT)) or lacking (LPIAT1^−/− (KO)) LPIAT1 were homogenized and lipids extracted from 0.5 mg wet weight as described in Materials and Methods. Targeted molecular species of PtdIns (A), PtdInsP (B), PtdInsP₂ (C) and PtdInsP₃ (D) were detected by MRM mass spectrometric analysis as described in Materials and Methods. Data are expressed as moles/mg protein, normalized to relevant internal standards. Shown are mean ± SD, n = 4 for both WT and KO. Data were analyzed by T-test. *p≤0.05, **p≤0.005.