Figure 5. Effect of LPIAT1 knockout on brain phospholipid molecular species.
Brains from 13 day old littermates expressing (LPIAT1+/+ (WT)) or lacking (LPIAT1−/− (KO)) LPIAT1 were homogenized and lipids extracted from 0.5 mg wet weight as described in Materials and Methods. Targeted molecular species of PC (A), PE (B), PS (C) and PA (D) were detected by MRM mass spectrometric analysis as described in Materials and Methods. Data are expressed as moles/mg protein, normalized to relevant internal standards. Shown are mean ± SD, n = 4 for both WT and KO. Data were analyzed by T-test. *p≤0.05, **p≤0.005.