Skip to main content
. 2013 Mar 5;8(3):e57672. doi: 10.1371/journal.pone.0057672

Figure 3. Confirmation of C. albicans FKS1-1 mutants with the S645Y mutation.

Figure 3

(A) Genomic DNA from SC5314 (wild-type), the FKS1-1 mutants (YC734 and YC736), and isolate 89 were PCR amplified with primers JC584/JC585 to detect a 410 bp (1810∼2220) product of the CaFKS1 gene, and then sequenced with primer JC584. Nucleotide 1934 of the CaFKS1 gene is labeled green. The C1934A mutation is present in FKS1-1 mutants derived from SC5314 and in the clinical isolate 89, but absent in SC5314. Nucleotide 1938 of the CaFKS1 gene is labeled purple. An A1938G silent mutation is present in the FKS1-1 mutants, but not in SC5314 or isolate 89. (B)&(C) The 410 bp PCR product of the CaFKS1 gene was amplified from genomic DNA from SC5314, FKS1-1 mutants (YC734 and YC736), and isolate 89, and digested with PflFI (B) or MseI (C) to confirm the C1934A or A1938G mutations are present and to confirm the mutations occurred in both alleles of the gene.