Figure 4. In vitro phenotypes and the in vivo efficacy of posaconazole combined with either caspofungin or FK506 against C. albicans FKS1-1 mutant.

Cells were grown overnight in YPD at 30°C, 5-fold serially diluted, spotted onto YPD medium containing an echinocandin (A) or an azole (B) at the concentrations indicated, and incubated at 37°C for 48 hr. The efficacy of posaconazole (2 mg/kg) and caspofungin (0.1 mg/kg) (C) or posaconazole (0.5 mg/kg) and FK506 (0.5 mg/kg) (D) against the C. albicans FKS1-1 mutant (YC734). The experimental procedures are similar to those described in the legend to Figure 1, except that the survival of the animals was monitored for up to 21 days.