Table 1. Inter-assay % Bias and precision (% CV) of lyso-Gb₃ quality control mouse tissue samples.

QC (Actual Conc.)		Kidney	Skin	Heart	Plasma
Low1(2.00 ng/mL)	Mean ± SD	2.16±0.306	2.02±0.233	2.12±0.122	2.15±0.210
	% CV	14.2	11.5	5.77	9.78
	% Bias	7.89	1.00	6.17	7.36
Low2(4.00 ng/mL)	Mean ± SD	4.10±0.327	4.18±0.517	4.23±0.413	4.09±0.616
	% CV	8.0	12.4	9.78	15.1
	% Bias	2.50	4.43	5.67	2.25
Mid(80.0 ng/mL)	Mean ± SD	84.9±8.43	82.3±5.51	92.5±3.91	79.8±12.6
	% CV	9.93	6.70	4.23	15.8
	% Bias	6.08	2.88	15.6	−0.250
High(160 ng/mL)	Mean ± SD	162±19.7	167±13.9	181±27.2	151±11.8
	% CV	12.2	8.34	15.0	7.83
	% Bias	1.32	4.50	13.2	−5.70

Quality control samples were prepared in wild-type mouse plasma or tissue homogenates at four concentration levels and analyzed on three separate days. Six to 10 replicates at each concentration level were used for inter-assay determination. Conc., concentration; SD, Standard Deviation.