Table 3. Recovery of lyso-Gb3 from plasma and tissue homogenates.
% Recovery (Mean) | |||||
Human | Mouse | ||||
Actual Conc.(ng/mL) | Plasma | Plasma | Skin | Heart | Kidney |
2 | 76 | 80 | 70 | 88 | 76 |
10 | 94 | 76 | 64 | 66 | 71 |
160 | 104 | 87 | 69 | 86 | 77 |
Recovery samples were prepared in normal human plasma, and wild-type mouse tissue homogenates or plasma at three concentration levels and analyzed in quintuplicates (n = 5). % recovery (mean) = 100×(mean peak area of extracted lyso-Gb3/mean peak area of unextracted lyso-Gb3).