Figure 4. Bcl-2 and NFκB peptides bind the ASPP2 n-src loop 1089–1097 peptide in a different site than p53CD.

(A) p53CD binds Fl-ASPP2 1089–1097 ASPP2 SH3 derived peptide In fluorescence anisotropy experiments with K _d = 0.63±0.02 µM (B) Non-labeled Bcl-2 or NFκB peptides were titrated into the fully formed complex between Fl-ASPP2 1089–1097 and p53CD. None of the peptides displaced p53CD from the complex.