Table 2. Effect of 10 mM malate on C. elegans ATP levels.
Strain | RNAi knockdown | ATP following 10 mM malate (% of same strain untreated) | Standard error |
N2 control | 60.4% | 3.3% | |
daf-16(mgDf50) | 38.7% | 1.8% | |
N2 | skn-1 | 62.4% | 1.9% |
N2 | sdha-2 | 64.4% | 4.1% |
hsf-1(sy441) | 84.5% | 3.0% | |
N2 | fum-1 | 88.4% | 2.7% |
N2 | gei-7 | 114.6% | 2.4% |
hif-1(ia4) | 118.2% | 2.5% | |
sir-2.1(ok434) | 149.9% | 2.8% | |
aak-2(ok524) | 166.0% | 5.1% |
Worms counted refers to the sum of the numbers counted on the first count day.
α-ketoglu = α-ketoglutarate. cPerformed in cell culture inserts.
ATP levels were measured as indicated in the Methods.