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. 2013 Mar 5;8(3):e58103. doi: 10.1371/journal.pone.0058103

Figure 5. Root meristem size decreases in 35Spro::IAA15 transgenic lines.

Figure 5

(A, B) A representative seedling with decreased root meristem size in 35Spro::IAA15 transgenic line (B) compared with that of the wild-type (A). Root meristem size was defined as the number of cortex cells between the cortex stem cell and the first elongated cortex cell (black arrows). (C, D) In five-day-old roots, meristem cell number is 18.04±2.88 (n = 30) in 35Spro::IAA15 transgenic lines contrasted with 32.00±2.51 (n = 30) in wild-type (C), ***P value <0.0001. In seven-day-old roots, meristem cell number is 20.14±4.24 (n = 30) in 35Spro::IAA15 transgenic lines in comparison with 34.53±3.02 (n = 30) in wild-type (D), ***P value <0.0001. (E, F) Double staining of Lugol’s solution and GUS in six-day-old seedlings of CycB1;1::GUS control (E) and CycB1;1::GUS/35Spro::IAA15 (F) indicates that the population of mitosis-dividing cells is decreased in the overexpression line. Black vertical lines represent the expression area of CycB1;1::GUS. (G, H) Overexpression of IAA15 leads to the repression of PLT1 as the fluorescence of PLT1::CFP is substantially decreased in PLT1::CFP/35Spro::IAA15 (H) contrasted with that in PLT1::CFP control (G). (I) Real time RT-PCR assay of PLT1 transcripts in roots of five-day-old wild-type and 35Spro::IAA15 transgenic lines. Data is presented as a mean ± SD from three independent assays, ***P value <0.0001. Bars: 50 µm.