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. 2013 Mar 5;8(3):e58103. doi: 10.1371/journal.pone.0058103

Figure 6. Over expression of IAA15 inhibits differentiation of stem cells.

Figure 6

(A, B) More cell layers are visualized in the root stem cell niche of five-day-old 35Spro::IAA15 (B) compared with that of the wild-type (A). (C) The stem cell layers (QC+CSC) of 35Spro::IAA15 is 3.10±0.68 (n = 39) compared with 2.45±0.1 (n = 22) in the control. The t test, ***P value = 0.0003. (D-G) To further confirm the developmental defects of the root stem cell niche, CSC or QC marker J2341 and WOX5::GFP were introduced into the 35Spro::IAA15 background and the progeny were further analyzed. The fluorescence of J2341 is detected in two cell layers in J2341/35Spro::IAA15 (E) in comparison with one cell layer in J2341 control (D), which indicates that one more cell layer observed in (B) is undifferentiated CSC. The fluorescence of WOX5::GFP is observed in the broader domain including cortex/endodermal initial cells of WOX5::GFP/35Spro::IAA15 (G) in comparison with two QC cells only in WOX5::GFP control (F). Stars in (G) and (F) mark the cells expressing WOX5::GFP. Red arrows in (A), (B), (D) and (E) indicate the putative QC while blue arrows indicate the putative CSC. Bars: (A, B) 20 µm; (D–G) 10 µm.