Table 1. Reduced basipetal and acropetal auxin transport in 35Spro::IAA15 transgenic lines.
IAA transport (fmols) | Control | 35Spro::IAA15 | P valuesa | |||||
−NPA | +NPA | P valuesb | −NPA | +NPA | P valuesb | −NPA | +NPA | |
Basipetal | 2.53±0.09 | 1.26±0.10 | <0.0001 | 1.05±0.14 | 0.87±0.07 | <0.0001 | <0.0001 | 0.23 |
Acropetal | 4.75±0.44 | 1.54±0.13 | <0.0001 | 2.03±0.10 | 1.34±0.06 | 0.28 | <0.0001 | 0.01 |
Basipetal and acropetal movement of IAA in roots was examined by applying [3H]-IAA in seven-day-old seedlings, and measuring radioactivity. Data are presented as mean ± SEM from 10 plants. P valuesa (control versus 35Spro::IAA15) and P valuesb (−NPA versus +NPA) were obtained by Student‘s t test.