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. 2013 Mar 5;8(3):e54643. doi: 10.1371/journal.pone.0054643

Figure 7. miR-27a targets both the seed regions of MCPH1 cloned together.

Figure 7

The luciferase reporter assay of different constructs of the full-length 3′-UTR of MCPH1 in KB cells. Note a significantly reduced luciferase activity in cells co-transfected with pMIR-Report-3′-UTR-S and pcDNA3/pre-miR-27a/EGFP (0.2 or 0.4 µg) in comparison to those with pMIR-Report or pMIR-Report-3′-UTR-AS, suggesting miR-27a targets MCPH1. Note that both the seed regions in the 3′-UTR of MCPH1 are important for the interaction with miR-27a as a significant increase in the luciferase activity was observed in cells transfected with pMIR-Report-3′-UTR-M1F, pMIR-Report-3′-UTR-M2F or pMIR-Report-3′-UTR-MF along with pcDNA3/pre-miR-27a/EGFP in comparison to cells transfected with pMIR-Report-3′-UTR-S and pcDNA3/pre-miR-27a/EGFP. Abbreviations: ns, statistically not significant; and, ***p<0.001.