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. 2013 Mar 5;8(3):e54643. doi: 10.1371/journal.pone.0054643

Figure 8. miR-27a targets both the seed regions of MCPH1 cloned separately.

Figure 8

(A) The luciferase reporter assay of different constructs harboring seed region 1 (SDR1) of MCPH1 3′-UTR in KB cells. Note a significantly reduced luciferase activity in cells co-transfected with pMIR-Report-3′-UTR-S1 and pcDNA3/pre-miR-27a/EGFP (0.2 or 0.4 µg) in comparison to those with pMIR-Report, suggesting miR-27a targets SDR1 of MCPH1. As expected, no significant difference in luciferase activity was observed in cells co-transfected with pMIR-Report-3′-UTR-AS1 or pMIR-Report-3′-UTR-M1 with pcDNA3/pre-miR-27a/EGFP (0.2 or 0.4 µg) in comparison to those with pMIR-Report. (B) The luciferase reporter assay of different constructs harboring seed region 2 (SDR2) of MCPH1 3′-UTR in KB cells. As with SDR1, miR-27a also targets SDR2.