Table 1. Comparison between multiparameter flow cytometry (MFC) and histopathology plus immunohistochemistry (IH) as regards identification of infiltration by neoplastic cells versus normal/reactive cells.
Diagnostic category | N. of concordant samples by MFC vs. IH/Total samples | (%) |
Non-infiltrated samples: | 17/17 | 100 |
Reactive/inflammatory samples | 9/9 | 100 |
Other non-infiltrated samples | 8/8 | 100 |
Infiltrated samples: | 33/35 | 94 |
Lymphomas: | 7/9 | 78 |
T-cell precursor LL | 2/2 | 100 |
B-cell precursor LL | 2/2 | 100 |
Diffuse large B cell lymphoma | 1/1 | 100 |
Burkitt lymphoma | 2/2 | 100 |
Hodgkin lymphoma | 0/1 | 0 |
Anaplastic lymphoma | 0/1 | 0 |
Solid Tumors: | 26/26 | 100 |
Neuroblastoma | 12/12 | 100 |
- Gaglioneuroblastoma | 1/1 | 100 |
Rhabdomyosarcoma | 4/4 | 100 |
Ewing Sarcoma/PNET | 2/2 | 100 |
Adrenal carcinoma | 1/1 | 100 |
Nasopharyngeal carcinoma | 1/1 | 100 |
Hemagiopericytoma | 1/1 | 100 |
Germ cell tumor | 3/3 | 100 |
Wilmstumor | 2/2 | 100 |
Total | 50/52 | 96 |
All 8 samples were obtained from pediatric cancer patients, but they were all negative for the presence of tumor cells by conventional diagnostic approaches. LL: lymphoblastic lymphoma; PNET- primitive neuroectodermal tumor.