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. 2013 Mar 5;8(3):e58393. doi: 10.1371/journal.pone.0058393

Table 1. The primers and site-directed mutable points of mutant keratinase genes.

Mutant gene Primer Sequences of primersa Mutable points
kerAopti1 kerAopti1F 5′-CCAGCTAAAAATGTTGAAAGGGA-3′ CCG/CCA (Pro-3); GCG/GCT (Ala-4)
kerAopti1R 5′-GAGCGGTACCAGCAATCC-3′
kerAopti2 kerAopti2F 5′-GTCAGAAACAGACTCTCCAGCAC-3′ CCG/CCA (Pro-3); GCG/GCT (Ala-4); CGC/AGA (Arg-322); CGT/AGA (Arg-324)
kerAopti1R 5′-TTGTGAAGCTGAAAGGTTCGGATG-3′
a

The mutable points are underlined.