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. 2013 Mar 5;8(3):e56929. doi: 10.1371/journal.pone.0056929

Figure 3. SPLUNC1 inhibited miR-141 expression and the effects of miR-141 and SPLUNC1 on PTEN expression in nasopharyngeal carcinoma cells.

Figure 3

(A) SPLUNC1 expression in CNE-1 nasopharyngeal carcinoma cells was higher than that in the HNE-2 and 5–8 F cells, while the miR-141 expression in CNE-1 cells was lower than that in the HNE-2 and 5–8 F cells (P<0.05). (B) When SPLUNC1 was overexpressed in HNE-2 and 5–8 F cells, miR-141 expression was decreased (P<0.05). (C) Expression of miR-141 mimics in NPC cells decreased the expression of PTEN; expression of miR-141 inhibitor increased the expression of PTEN. (D) Enforced expression of SPLUNC1 in NPC cells increased PTEN expression. (E) miR-141 mimics inhibited PTEN expression and increased Akt phosphorylation in the presence of enforced SPLUNC1-expression in NPC 5–8 F cells. (F) Enforced expression of SPLUNC1 decreased Akt expression in NPC cells; and decreased the levels of phosphorylated Akt and MDM2 (G), but had little effect on GSK3β. SPLUNC1 inhibited the baculovirus phosphatase (BVP)-induced phosphorylation of PTEN and Akt(H).