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. 2013 Mar 5;8(3):e57929. doi: 10.1371/journal.pone.0057929

Figure 1. Npy induction within visceral fat and SVF with diet-induced obesity.

Figure 1

Npy gene expression by quantitative RT-PCR in (A) epididymal fat (EWAT) and (B) inguinal fat depots (IWAT) of normal diet (ND), 8 week high fat diet (HFD), and 20 week HFD C57Bl6 mice. N = 4 mice per group. (C) Npy expression in various fat depots of ND and 16 week HFD fed animals. N = 4 mice per group. (D) Npy expression in stratified adipose tissue. EWAT from HFD C57Bl6 mice was separated into adipocyte (Ad) and stromal vascular fraction (SVF) cells after collagenase digestion. Npy expression was assessed by quantitative RT-PCR. N = 4 per group. (E) Npy1r and Npy2r expression in adipocytes and SVF cell fractions from lean mice. N = 4 per group. (F) NPY protein expression in cultured SVF cells from HFD C57Bl6 mouse EWAT and IWAT. Media was removed from cells after 18 hours of culture and assessed for NPY by ELISA (N = 3 wells per group). *p<0.05, **p<0.01,***p<0.005 by t-test.