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. 2013 Mar 5;8(3):e57929. doi: 10.1371/journal.pone.0057929

Figure 6. NPY treatment decreases circulating Ly6c+ monocyte and CD11c+ ATM content.

Figure 6

Lean C57Bl/6 mice were treated with NPY or NPY scramble control peptide (60 µg/kg/day) IP for 10 days. N = 4 mice per group. (A) Quantitiation of ATMs by flow cytometry. EWAT SVF cells were stained for ATM markers (F4/80, CD11b, CD11c) and analyzed by flow cytometry. ATM content normalized to fat pad weight. Gene expression analysis of EWAT for (B) inflammatory genes by quantitative RT-PCR. (C) light microscopy image showing larger adipocytes with NPY injection (left panel) compared to scramble control peptide injection (right panel) (D) Gene expression analysis of EWAT for adipocyte genes by quantitative RT-PCR. *p<0.05. (E) Quantitation of Ly6chi and Ly6clo CD115+ blood monocytes by flow cytometry (N = 4 per group). Data is representative data of one of 3 replicate experiments.